High sucrose and agar is apt to keep the tight close structure of callus . ( 4 ) the optional inducing medium of adventitious buds is ms basal medium with 2mg / l 6 - ba , 30g / l sucrose and 9g / l agar . all the tested genotypes have high inducing rate on this medium 供試的4個基因型在此培養(yǎng)基上不定芽誘導效率均最高,由高到低依次為:松93 - 8為962 . 9 ;東農v10為679 . 7 ;東農419為542 . 5 ;富士光為482 . 6 ,與其它處理差異達到顯著。
The results showed that high concentration of 2 , 4 - d was required for callus induction from mature seeds of tall fescue , and combination of 8mg / l 2 , 4 - d with 2mg / l aba gave best induction effects . by slicing sterilized seeds longitudinally or cutting embryos , callus induction frequency was profoundly increased over intact seeds from one and half to eight times . adoption of ms basal medium and supplementation of 0 . 5g / l casamino acids and 0 . 5g / l glutamine in medium were found to help to facilitate callus induction 研究表明,高羊茅成熟種子愈傷組織誘導需要較高濃度的2 , 4 - d ,以8mg l2 , 4 - d與2mg laba配合能獲得最佳的誘導效果;種子滅菌后縱切或切胚,可使出愈率成倍提高;采用ms基本培養(yǎng)基和在培養(yǎng)基中添加0 . 5g l的水解酪蛋白與谷氨酰胺也有助于提高出愈率;低劑量( 10gy )射線輻照處理對成熟種子愈傷組織尤其是胚性愈傷組織形成有一定的刺激效應。
2 established the regeneration system with mature embryos of rice ( 1 ) the optional inducing medium of callus is ms basal medium supplemented 3mg / l 2 , 4 - d , 30g / lsucrose and 8g / l agar . ( 2 ) the rate of callus induction varied with the genotypes . dongnong v - 10 has the highest inducing rate among 4 tested genotypes , up to 96 . 1 % ; the second is song 93 - 8 and dongnong 419 , and the average inducing rate renched 88 . 9 % and 88 . 3 % respectively ( 2 )不同基因型愈傷組織誘導率有差異,供試的4個基因型中,東農v10誘導率最高,平均誘導率達到96 . 1 ;其次是松93 - 8和東農419 ,平均誘導串分別為88 . 9和88 . 3 ;富士光誘導率最低,平均誘導率為78 . 9 。
The regeneration system of tobacco was established . the adventitious shoots were induced from leaf explants of tobacco based on ms basal medium supple - - mented with 2 . 0mg / l 6 - ba and 0 . 3mg / l naa . then the regenerated plants were rooted on ms medium containing 0 . 3mg / l naa 植株再生體系的建立采用無菌苗煙草葉片為外植體,以ms為基本培養(yǎng)基,篩選出ms + 6 - ba2 . 0mg l + naa0 . 3mg l以誘導不定芽的分化,并于ms + naa0 . 3mg l的pa碩士學位論文v沉了砂“工隊nk ”主根培養(yǎng)基上生根,獲得完整再生植株。
( 1 ) plant regeneration system of cucumber contyledonary node was established . ( 1 ) the cotyledonary node was regarded as optimal explant by comparing the differentiation frequency of 3 kinds of explants ( cotyledon , cotyledonary node and hypocotyl ) . ( 2 ) by discussing the effect of pgr ( naa and 6 - ba ) concentration and combination on differentiation frequency of adventitious buds , we concluded that the optimal shoot induction medium is ms basal medium with 1mg / l 6 - ba ( ph5 . 8 ) ( 1 )黃瓜子葉節(jié)植株再生建立東北農業(yè)大學理學碩士學位論文一通過對比三種外植體(于葉、子葉節(jié)、下胚軸)的分化率,得出最佳外植體類型為子葉節(jié);通過探討pgr ( naa 、 6 ba )濃度和配比對不定芽分化的影響,得出最佳芽誘導培養(yǎng)基為:卜1叭6 。